AllPhase human MSC culture medium
A complete xeno-free culture medium for growth and expansion of hMSCs
‧ Complete xeno-free medium for hMSCs
‧ Superior expansion at high cell densities
‧ Suit for long-term growth and differentiation potential
‧ Consistent manufacture and quality control
‧ Flexible and compatible with adhesion system (e.g. Fibronectin, Gelatin and CellBIND plate)
Redefining quality culture and advancing clinical therapy
AllPhase human MSC culture medium is a complete xeno-free medium for supporting the growth and expansion of human mesenchymal stem cells (hMSCs), including adipose derived stem cell (ADSC) and umbilicord derived mesenchymal stem cell (UC-MSC).
hMSCs cultured in AllPhase human MSC culture medium show superior expansion and self-renewal potency in comparison to serum-containing medium and other commercial serum-free medium (Fig.1). Also, these hMSCs exhibit a primitive morphology(Fig.2), tri-lineage differentiation potential (Fig.3)and normal hMSC marker profiles over long-term culture (Fig.4).
Figure 1. AllPhase human MSC culture medium enables excellent poliferation of hMSCs. The equivalent study shows AllPhase culture medium has the better performance of poliferation compared with serum-containing medium and other commercial serum-free medium.
Figure 2. hMSCs primitive phenotype.
Human MSCs grow in AllPhase MSC culture medium exhibit a primitive morphology, which is a much smaller, spinle-shaped morphology and can reach densities >1.0×10^5 cells/cm^2.
Figure 3. hMSCs cultured in AllPhase MSC culture medium retain strong differentiation potential. Human ADSCs differentiate into osteoblasts (Alizarin red S staining), adipocyte (Oil red O staining) and chondrocyte (Alcian Blue staining).
Figure 4. Flow cytometric analysis of hMSCs cultured in AllPhase MSC culture medium. Human ADSCs differentiate from passage 5 were stained for mesenchymal cell surface markers CD90, CD44, CD105, CD73 and the MSC negative marker (MSC-).