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AllPhase human MSC culture medium

A complete medium ideal for supporting the growth and
expansion of human mesenchymal stem cells

  • Xeno-free
  • Suitable for BM-MSC, ADSC, and UC-MSC
  • Doubling time < 30h (P1-P5)
  • Coating-free
  • ISO13485 certified
  • GMP grade
Name Cat. # Size
AllPhase human MSC culture kit
AllPhase Supplement 20X (GMP grade)
AML500
AGA1000-S
500 ml
50 ml

AllPhase human MSC culture medium is a complete xeno-free medium supporting the growth and expansion of human mesenchymal stem cells (hMSCs), including bone marrow mesenchymal stem cell (BM-MSC) adipose derived stem cell (ADSC) and umbilical cord derived mesenchymal stem cell (UC-MSC). Duogenic AllPhase medium makes cells expansion more efficient without any coating. Each AllPhase MSC culture medium lot is ISO13485 certified, GMP compliant. and controlled by rigorous quality procedure.

AllPhase human MSC culture medium enables an excellent proliferation of hMSCs. AllPhase culture medium has a better performance of proliferation compared to serum-containing medium and other commercial serum-free medium.

 

 

hMSCs cultured with AllPhase medium retain a strong differentiation potential. Human ADSCs differentiate into osteoblasts (Alizarin red S staining), adipocyte (Oil red O staining)and chondrocyte (Alcian Blue staining).

 

Flow cytometry analysis of hMSCs in AllPhase medium. Human ADSCs differentiate after passage 5 and were stained with the surface markers CD90, CD44, CD105, CD73 and the MSC negative markers (MSC-).

 

 

 

Coating free adhesion capacity. Human ADSCs cultured in AllPhase have similar adhesion capacity than cells cultured in serum-containing medium. Cells were stained with crystal violet 20, 40 and 60 minutes after seeding.

 

 

 

 

AllPhase is suitable for 3D culture. Human ADSCs can be expanded under 3D culture condition without microcarrier when cultured with AllPhase MSC culture medium.