Pluto human iPS/ES cell culture medium

A chemically defined, serum-free, xeno-free culture medium for human iPS/ES cells

‧ Chemically defined, serum-free, xeno-free
‧ Complete, ready-to-use
‧ Efficient proliferation of undifferentiated ES and iPS cells
‧ Stable pluripotency over long-term culture
‧ Consistent manufacture and quality control
‧ Flexible and compatible with multiple coating (e.g. Matrigel, Geltrex and Vitronectin)

Make the culture well-controlled

Pluto human iPS/ES cell culture medium is a chemically defined, serum-free, xeno-free medium designed to support the growth and expansion of human induced pluripotency stem cell (iPSC) and embryonic stem cell (ESC) in a feeder-free culture system. Pluto human iPS/ES cell culture medium offers the ability to maintain pluripotent cells without containing a high level of bFGF and other growth factors or cytokines. The succinct formulation contains the most essential components required for the maintenance of human iPSC and ESC, and maintain the full differentiation potential of pluripotent stem cells.

Each Pluto human iPS/ES cell culture medium lot is made according to a consistent manufacturing procedure and complies with rigorous quality controls ensuring a stable performance and a reliable cellular behavior.

PLUTO FIG1

Figure 1. Pluto human iPS/ES cell culture medium enables an excellent poliferation of hES cells. The following study demonstrates that the proliferation performance of Pluto human iPS/ES cell culture medium is similar to the commercial product.

Normal cell morphology and pluripotency performance assurance

The hESC and hiPSC cultured in Pluto human iPS/ES cell culture medium without feeder layer show compact colonies with a high nucleus-to-cytoplasm ratio, prominent nucleoli, and discernible colony borders, characteristics of healthy undifferentiated pluripotent stem cells (Fig.2). Furthermore, these pluripotent stem cells express important pluripotent markers and keep their potential to differentiate into the three germ cell layers, i.e., endoderm, mesoderm, and ectoderm. The pluripotency markers expression is analyzed by flow cytometry (Fig.3) and the differentiation potential is assessed by  embryoid bodies formation in vitro and teratomas formation in vivo (Fig.4).

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Figure 2. Normal colony morphology.
The H9 hES cells cultured in Pluto human iPS/ES cell culture medium on Geltrex-coated plates display a colony morphology typical of normal feeder-free iPS/ES cell cultures, including high nucleus-to-cytoplasm ratio, prominent nucleoli, and distinct colony edges.

Figure 3. Human iPS cells cultured in Pluto human iPS/ES cell culture medium maintain a high expression level of pluripotency markers. The flow cytometry results show that the pluripotency maintenance in similar in Pluto human iPS/ES cell culture medium and commercial media products in feeder and feeder-free culture systems.

PLUTO FIG3
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Figure 4. Differentiation potential assessed by inducing embryoid body formation (Top and Middle) and teratomas formation (Bottom).

Arrow: Gland; Arrow head: Bone; Star: Melanocyte