Pluto human iPS/ES cell culture medium

A chemically defined, serum-free, xeno-free culture medium for human iPS/ES cells

‧ Chemically defined, serum-free, xeno-free
‧ Completely, ready-to-use
‧ Efficient proliferation of undifferentiated ES and iPS cells
‧ Stable pluripotency over long-term culture
‧ Consistent manufacture and quality control
‧ Flexible and compatible with multiple matrices (e.g. Matrigel, Geltrex and Vitronectin)

Make the culture under well-control

Pluto human iPS/ES cell culture medium is a chemically defined, serum-free, xeno-free medium designed to support the growth and expansion of human induced pluripotency stem cell (iPSC)and embryonic stem cell (ESC) in a feeder-free culture system. Pluto human iPS/ES cell culture medium offers the ability to maintain pluripotent cells without high levels of bFGF and other growth factors or cytokines. The succinct formulation contains the most essential components required for maintenance of human iPSC and ESC, providing a simplified medium and keep the full differentiation potential of pluripotent stem cells.

Each lot of Pluto human iPS/ES cell culture medium, made by consistent manufacture procedure and rigorous quality control, provides stable performance and reliable cellular behavior.

PLUTO FIG1

Figure 1. Pluto human iPS/ES cell culture medium enables excellent poliferation of hES cells. The equivalent study demonstrates Pluto human iPS/ES cell culture medium has the same performance of poliferation with commercial product.

Normal cell morphology and performance assurance of pluripotency

The hESC and hiPSC cultured in Pluto human iPS/ES cell culture medium without feeder layer show the compact colonies with a high nucleus-to-cytoplasm ratio, prominent nucleoli, and distinct colony borders which are characteristic morphology of healthy undifferntiated pluripotent stem cells, and can be observed through a phase-contrast microscope (Fig.2). Also, these pluripotent stem cells maintain expression of the important pluripotent markers and hold the potential to differentiate into cell type of all three germ layer, i.e., endoderm, mesoderm, and ectoderm. The pluripotent markers expression is analyzed by flow cytometry (Fig.3) and differentiation potential is assessed by specific treatment with embryoid body cultured in vitro and teratomas in vivo (Fig.4).

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Figure 2. Normal colony morphology.
The H9 hES cells cultured in Pluto human iPS/ES cell culture medium on Geltrex coated plates display colony morphologies typical of normal feeder-free iPS/ES cell cultures, including high nucleus-to-cytoplasm ratio, prominent nucleoli, and distinct colony edges.

Figure 3. Human iPS cells cultured in Pluto human iPS/ES cell culture medium maintain highly expression level of pluripotent markers.The equivalent study demonstrates Pluto human iPS/ES cell culture medium has the same ability of pluripotent maintenance with commercial products in feeder and feeder free culture system.

PLUTO FIG3
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Figure 4. Differentiation potential assessed by induction with embryoid body (Top and Middle) and teratomas formation (Bottom).

Arrow: Gland; Arrow head: Bone; Star: Melanocyte